Hemoglobin Brockton [beta 138 (H16) Ala—-Pro] has very low oxygen affinity but electrophoretic mobility and hemolysate binding are normal, suggesting that the substitution at beta 138 won't disrupt important polar interactions, like the hydrogen bonds and salt bridges involving Asp 137 and Professional 138, that contribute for the cooperative oxygen-binding https://nicolaslecourtmansion.com